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zetaprobe gt membrane  (Bio-Rad)


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    Structured Review

    Bio-Rad zetaprobe gt membrane
    Zetaprobe Gt Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zetaprobe+gt/Zeta-Probe+GT+Membrane/pm41418758-435-6-9
    Average 96 stars, based on 1714 article reviews
    zetaprobe gt membrane - by Bioz Stars, 2026-09
    96/100 stars

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    Electrophoresis:

    Article Title: Angiotensin II induces skeletal muscle wasting through enhanced protein degradation and down-regulates autocrine insulin-like growth factor I.
    Article Snippet: Northern blot analysis and solution hybridization/ribonuclease (RNase) protection assay Total RNA was prepared from frozen liver or gastrocnemius muscle using Tri-Reagent (Molecular Research Center, Inc., Cincinnati, OH) and was assessed for purity by measuring absorptions at 260 and 280 nm. .. Total RNA (20 mg) was separated by electrophoresis in a 1% agaroseformaldehyde gel, transferred to ZetaProbe GT (Bio-Rad Laboratories, Inc., Hercules, CA) or Hybond C (Amersham Pharmacia Biotech, Arlington Heights, IL) membrane, and cross-linked to the membrane by UV irradiation. ..

    Membrane:

    Article Title: Angiotensin II induces skeletal muscle wasting through enhanced protein degradation and down-regulates autocrine insulin-like growth factor I.
    Article Snippet: Northern blot analysis and solution hybridization/ribonuclease (RNase) protection assay Total RNA was prepared from frozen liver or gastrocnemius muscle using Tri-Reagent (Molecular Research Center, Inc., Cincinnati, OH) and was assessed for purity by measuring absorptions at 260 and 280 nm. .. Total RNA (20 mg) was separated by electrophoresis in a 1% agaroseformaldehyde gel, transferred to ZetaProbe GT (Bio-Rad Laboratories, Inc., Hercules, CA) or Hybond C (Amersham Pharmacia Biotech, Arlington Heights, IL) membrane, and cross-linked to the membrane by UV irradiation. ..

    Article Title: Tissue-Specific Splicing of the Herpes Simplex Virus Type 1 Latency-Associated Transcript (LAT) Intron in LAT Transgenic Mice
    Article Snippet: .. RNA was transferred to a ZetaProbe GT (Bio-Rad) nylon membrane according to the manufacturer's instructions. .. Hybridization was performed with random hexamer-primed (random labeling kit; Roche) [ 32 P]dCTP-labeled pATD19 probe (see below) to detect expression of the 2.0-kb LAT intron.

    Article Title: Tissue-Specific Splicing of the Herpes Simplex Virus Type 1 Latency-Associated Transcript (LAT) Intron in LAT Transgenic Mice
    Article Snippet: .. RNA was transferred to a ZetaProbe GT (Bio-Rad) nylon membrane according to the manufacturer’s instructions. .. Hybridization was performed with random hexamer-primed (random labeling kit; Roche) [32P]dCTP-labeled pATD19 probe (see below) to detect expression of the 2.0-kb LAT intron.

    Article Title: N-glycosylated molecules act as a co-precipitant in RNA purification
    Article Snippet: .. For nylon membrane, ZetaProbe GT™ from Bio-Rad™ was used. ..

    Irradiation:

    Article Title: Angiotensin II induces skeletal muscle wasting through enhanced protein degradation and down-regulates autocrine insulin-like growth factor I.
    Article Snippet: Northern blot analysis and solution hybridization/ribonuclease (RNase) protection assay Total RNA was prepared from frozen liver or gastrocnemius muscle using Tri-Reagent (Molecular Research Center, Inc., Cincinnati, OH) and was assessed for purity by measuring absorptions at 260 and 280 nm. .. Total RNA (20 mg) was separated by electrophoresis in a 1% agaroseformaldehyde gel, transferred to ZetaProbe GT (Bio-Rad Laboratories, Inc., Hercules, CA) or Hybond C (Amersham Pharmacia Biotech, Arlington Heights, IL) membrane, and cross-linked to the membrane by UV irradiation. ..

    Isolation:

    Article Title: Angiogenesis modulators expression in culture cell lines positives for HPV-16 oncoproteins.
    Article Snippet: mediate the oncogenic transformation of squamous epi a i 7 t t G G c t s e i a s a i ( e h a i d a p t d f e w t o s p c i y e u H Altered angiogenesis response is observed in paients with cervical cancer.. In this study we examined hether Human Papilloma Virus (HPV) positive epihelial cells are able to produce angiogenic modulaors.. When added to human umbilical vein endothelial ells (HUVEC) the media conditioned by HPV-16 posiive cells was able to induce proliferation, whereas a ontrary effect was observed for media derived from on-tumorigenic keratinocytes.

    Article Title: The La protein functions redundantly with tRNA modification enzymes to ensure tRNA structural stability
    Article Snippet: .. For Northern analyses, 5 μg of RNA was fractionated in 5% polyacrylamide / 8 M urea gels and transferred to ZetaProbe GT (Bio-Rad) in 0.5 TBE at 150 mA for 16 h. For aminoacylation analyses, RNA was isolated by lysing cells in pH 4.5 phenol ( Varshney et al. 1991 ). ..

    Northern Blot:

    Article Title: The La protein functions redundantly with tRNA modification enzymes to ensure tRNA structural stability
    Article Snippet: .. For Northern analyses, 5 μg of RNA was fractionated in 5% polyacrylamide / 8 M urea gels and transferred to ZetaProbe GT (Bio-Rad) in 0.5 TBE at 150 mA for 16 h. For aminoacylation analyses, RNA was isolated by lysing cells in pH 4.5 phenol ( Varshney et al. 1991 ). ..



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